Composite

Part:BBa_K2328058:Design

Designed by: Yiyuan Gao   Group: iGEM17_TJU_China   (2017-10-20)


HU + Linker I + nonsense sequence + Linker II +Linker.a + smURFP III + Histag.a + pMB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 131
    Illegal XhoI site found at 125
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 566
    Illegal NgoMIV site found at 643
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 194
    Illegal SapI site found at 1729


Design Notes

for dream


Source

arashi

References